Имеют ли экспериментальные мРНК-вакцины долгосрочные последствия?

COVID-19 mRNA-based vaccines have used codon optimization to improve protein production. A codon consists of three nucleotides and these are the building blocks of DNA.

Codon optimization guarantees unexpected results

Replacement of rare codons must be done sensibly, as rarer codons may have slower transformation rates and, in fact, a slower rate is necessary to avoid misfolded proteins.

Stop (or termination) codons, when present at the end of an mRNA coding sequence, signal the end of protein synthesis. According to a recent article, both Pfizer and Moderna selected suboptimal stop codons.

The anti-COVID vaccines stimulate the spike protein (Spike) to levels unheard of in nature, and unfortunately the Spike protein is the toxic part of the virus that is responsible for the most harmful effects of the virus, such as blood clotting disorders, neurological problems, and heart damage. To expect that the COVID vaccine would not produce these kinds of effects would be naïve.

Other major threats include immune dysfunction and the outbreak of latent viral infections such as shingles and herpes. Co-infections, in turn, could accelerate other diseases. Herpes viruses, for example, have been cited as a cause of both AIDS and chronic fatigue syndrome.

Anti-COVID vaccines use codon optimization

A Twitter user named Ehden wrote the following, “Let’s start with a thought experiment: if there is an engineering design flaw and no one considers it, can it actually harm people or kill them? ” He says there is an overlooked aspect of mRNA vaccines against COVID, something called “codon optimization,” that virtually guarantees unexpected results.

Ehden explains:

“Trying to tell your body to generate proteins is difficult for many reasons. One of them is the fact that when you try to run the protein information through the ribosomes that process that code and generate the protein, it can be a slow process or it can get stuck for quite a while.

Luckily, scientists discovered a way to overcome this problem by replacing the code: instead of using the original genetic code to generate the protein, they changed the letters in the code to optimize the code. This is known as codon optimization.”

A codon consists of three nucleotides. Nucleotides are the building blocks of DNA. An August 2021 article, published in the journal Nature Reviews Drug Discovery, addressed the use of codon optimization as follows:

“The open reading frame of the mRNA vaccine is the most important component, because it contains the coding sequence that is translated into protein.”

CVnCoV vaccine candidates against SARS-CoV-2

Although the open reading frame is not as malleable as the noncoding regions, it can be optimized to increase transformation without altering the protein sequence by replacing occasionally used codons with more frequently encountered codons that code for the same amino acid residue.

For example, the biopharmaceutical company CureVac AG discovered that human mRNA codons rarely have an A or U codon in the third position, and patented a strategy that replaces the A or U codon in the third position in the open reading frame with G or C. CureVac used this optimization strategy for its candidate CVnCoV vaccine against SARS-CoV-2.

Although rare codon replacement is an attractive optimization strategy, it should be used judiciously. This is because, for some proteins, the slower transformation rate of rare codons is necessary to achieve well-folded proteins.

To maximize transformation, the mRNA sequence incorporates modified nucleosides, such as pseudouridine, N1-methylpseudouridine or other nucleoside analogues. Because all native mRNAs include modified nucleosides, the immune system evolved to recognize unmodified single-stranded RNA, which is a hallmark of viral infection.

Unmodified mRNA specifically is recognized by pattern recognition receptors, such as Toll-like receptor 3 (TLR3), TLR7 and TLR8, and retinoic acid-inducible gene I receptor (RIGI). TLR7 and TLR8 receptors bind to guanosine- or uridine-rich regions in the mRNA and cause the production of type I interferons, such as IFNα, which can block mRNA transformation.

The use of modified nucleosides, in particular modified uridine, prevents recognition of pattern recognition receptors, allowing good levels of transformation to produce prophylactic amounts of protein.

Different types of optimization

Both Moderna’s and Pfizer – BioNTech’s SARS-CoV-2 vaccines contain nucleoside-modified mRNA. Another strategy to avoid detection by pattern recognition receptors that CureVac initiated uses sequence engineering and codon optimization to reduce uridines by increasing the GC content of the vaccine mRNA.”

According to Ehden, 60.9% of the codons in the anti-COVID vaccines have been optimized, which is equivalent to 22.5% of the nucleotides, but he does not specify which vaccine he is talking about or where the data came from.

However, it is clear that all anti-COVID mRNA vaccines use codon optimization to one degree or another. An article published in July 2021,4 in the journal Vaccines, evaluates the Pfizer/BioNTech and Moderna mRNA vaccines, as well as noting the following:

“The design of the Pfizer/BioNTech and Moderna mRNA vaccines involves different types of optimizations. Vaccine mRNA components must have a 5′-UTR to efficiently load ribosomes into the mRNA to initiate transformation, use optimal codons for further transformation, and an optimal stop codon to effectively terminate transformation.

Both the 5′-UTR and the 3′-UTR downstream must be optimized to achieve mRNA stability. The replacement of uridine with N1-methylpseudourinine (Ψ) complicates some of these optimization processes because Ψ is more versatile than U. Different optimizations may conflict with each other and some tinkering would be necessary.

I highlight the similarities and differences between the Pfizer/BioNTech and Moderna mRNA vaccines, while discussing the advantages and disadvantages of each to facilitate future vaccine improvement. In particular, I point out some optimizations in the design of the two mRNA vaccines that have not been done correctly.”

What can go wrong?

A key conclusion from the Nature Reviews Drug Discovery article we discussed earlier is that rare codon substitution “must be done sensibly,” as rarer codons may have slower transformation rates and a slower rate is necessary to prevent misfolded proteins.The Spike protein is the toxic part of the virus and that is also responsible for the most deleterious effects, such as blood clotting disorders, neurological problems, and heart damage. To expect that the anti-COVID vaccine would not produce these kinds of effects would be very naive.

The A (adenine) and U (uracil) codes in the third position are rare, whereas the anti-COVID vaccines replace the A and U codes with G (guanine) or C (cytosine). This change results in a 1000-fold higher amount of Spike protein compared to the actual virus infection.

Just about anything

What could go wrong? Well, just about anything. Again, the vaccine produces the Spike protein at levels unheard of in nature (even if SARS-CoV-2 is a “trick” artificial concoction), and this protein is the toxic part of the virus that is responsible for the most deleterious effects, such as blood clotting disorders, neurological problems, and heart damage.

Therefore, to expect that the anti-COVID vaccine would not produce these kinds of effects would be very naive. Codon changes can also cause protein misfolding, which is also bad news. As Dr. Stephanie Seneff explained in one of our interviews:

“The Spike proteins that these mRNA vaccines produce can’t get into the membrane, which I think is going to encourage it to become a prion protein that will give problems. Then, when it becomes inflamed, it upregulates alpha-synuclein [a neuronal protein that regulates synaptic trafficking and neurotransmitter release].

Then, alpha-synuclein will embed itself in misfolded Spike proteins, which causes a big problem inside the dendritic cells in the germinal centers of the spleen. And that will collect all this junk into exosomes and release them. Then they will travel along the vagus nerve to the brain stem and cause problems like Parkinson’s disease.

And maybe it will cause people who are not prone to Parkinson’s disease to get it, especially if they get the vaccine every year. Every year that you get a booster, you’ll get closer to the date for getting Parkinson’s disease.”

Immune dysfunction and viral outbreaks

Other major threats include immune dysfunction and the outbreak of latent viral infections, something Dr. Judy Mikovits has long warned about:

“We use poly(I: C) [a toll-like receptor 3 agonist] to tell the cell to activate the type I interferon pathway, and because [the spike protein your body produces in response to the anti-COVID vaccine] is not a natural synthetic envelope, there will be no poly(I: C), and it doesn’t [activate] the type I interferon pathway.

It ignored the plasmacytoid dendritic cell, which combined with IL-10, by talking to regulatory B cells, decides which antibody subclasses to eliminate. That means it also ignored the communication between the innate and adaptive immune response. Consequently, it missed endocannabinoid receptor signaling.

A big part of Dr. [Francis] Ruscetti’s and my work over the last 30 years has been to show that you don’t need a transmissible infectious virus, just fragments and parts of these viruses, because they also activate danger signals. They act as danger signals and pathogen-related molecular patterns.

So you synergistically give up that inflammatory cytokine signature that makes your innate immune response out of control. It can’t keep up with myelopoiesis [the production of cells in the bone marrow]. So there is a gap between mesenchymal stem cells and TGF-beta-regulated hematopoietic stem cells.

This means you could see bleeding disorders at both ends. Your defenses are insufficient. Your innate immune response can’t get there, so you will have a total disaster in your immune system.”

We now see reports of shingles and shingles infection after the anti-COVID-19 vaccine, and this is just what you can expect if your type I interferon pathway is disabled. However, that’s not all the problems, as these co-infections could also accelerate other diseases.

For example, herpes viruses have been listed as causing AIDS and myalgic encephalomyelitis (chronic fatigue syndrome or ME-CFS). According to Mikovits, these diseases do not appear until viruses from different families are related and retroviruses eliminate the type 1 interferon pathway. In the long term, the massive anti-COVID vaccine campaign could set the stage for a cascade of debilitating chronic diseases.

Are the anti-COVID vaccines optimized correctly?

As noted in the Vaccines article I cited earlier, optimizing the codons in the Pfizer and Moderna vaccines could be a big problem:

“As mammalian host cells attack exogenous unmodified RNA, all U nucleotides are replaced by N1-methylpseudouridine (Ψ). However, Ψ oscillates more in base pairing than U and can pair with A and G, and also, to a lesser extent, with C and U.

This is likely to increase the misreading of a codon by a near cognate tRNA. When the nucleotide U in the termination codons was replaced by Ψ, it increased the rate of misreading of a termination codon by a near cognate tRNA.

Such read-through events would not only decrease the amount of immunogenic proteins, but also produce a longer protein of unknown fate with potentially deleterious effects.

The designers of both vaccines considered CGG as the optimal codon in the CGN codon family and recoded almost all CGN codons to CGG. Many lines of evidence suggest that CGC is a better codon than CGG. The designers of the mRNA vaccines (especially mRNA-1273) chose an incorrect codon as the optimal codon.”

The paper also points out the importance of the vaccine mRNA being transformed accurately and not just effectively, because if the wrong amino acids are incorporated, it can confuse your immune system and prevent it from identifying the correct targets.

Accuracy is also important in transformation, and here it is a matter of selecting the correct stop codons. Stop codons (UAA, UAG or UGA), when present at the end of an mRNA coding sequence, signal the termination of protein synthesis.

According to the author, both Pfizer and Moderna selected stop codons that are suboptimal. “UGA is a poor choice of stop codons, and UGAU in the Pfizer/BioNTech and Moderna mRNA vaccines could be worse,” she says.

What health problems will be most common?

Although the range of diseases where we could see an increase as a result of this vaccine campaign is very high, some general predictions can be made. We have already seen more cases of blood clotting disorders, heart attacks and strokes, as well as cardiac inflammation.

In the long term, Seneff believes we will also see more cases of cancer, accelerated Parkinson’s-like diseases, Huntington’s disease and all kinds of autoimmune diseases and neurodegenerative disorders.

Mikovits also suspects that many people will develop chronic and debilitating diseases, as well as die prematurely. He also considers those who are asymptomatically infected with XMRV and gammaretroviruses from contaminated conventional vaccines to be at increased risk. The COVID vaccine will hasten their death by crippling their immune function. “Vaccinated kids are ticking time bombs,” Mikovits said in my May 2021 interview.

What are the options?

While this is all very problematic, there is still hope. I believe the best thing you can do is to develop your innate immune system. To do that, you need to be metabolically flexible and optimize your diet. You also need to make sure your vitamin D level is between 60 ng/mL and 80 ng/mL (100 nmol/L to 150 nmol/L).

I recommend time-restricted eating, which is a regimen where you eat all of your meals for the day within a six- to eight-hour period. Time-restricted eating will up-regulate autophagy, which may help digest and eliminate the Spike protein. Avoid all vegetable oils and processed foods. Focus on certified organic foods to minimize your exposure to glyphosate.

Sauna therapy can also be very effective, as it up-regulates heat shock proteins, which can help refold misfolded proteins. It also attacks damaged proteins and eliminates them.